Review



ethidium homodimer-1 (ethd-1)  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher ethidium homodimer-1 (ethd-1)
    Ethidium Homodimer 1 (Ethd 1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/ethidium+homodimer+1/pm40662251-207-6-12
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-1 (ethd-1) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Ethidium Homodimer Assay:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.

    Staining:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.

    Concentration Assay:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.

    Incubation:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.

    Blocking Assay:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.

    MTS Assay:

    Article Title: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.
    Article Snippet: The live cells were visualized with 0.1 mM Calcein-AM (emission at 488 nm) and dead cells with 0.5 mM ethidium homodimer-1 (EthD-1, emission at 568 nm, both from Thermo Fisher Scientific).

    Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
    Article Snippet: Ethidium homodimer-1 (EthD-1; Thermo Fisher Scientific) was added to a concentration of 1 μg/mL.

    Article Title: Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
    Article Snippet: LIVE/DEAD staining was carried out using Calcein AM (Thermo Fisher Scientific) and ethidium homodimer-1 (EthD-1, Invitrogen) solutions.

    Article Title: Bioactive and chemically defined hydrogels with tunable stiffness guide cerebral organoid formation and modulate multi-omics plasticity in cerebral organoids.
    Article Snippet: The viability and proliferation of cells growing n HA-Tyr (1 and 3 % wt.) and HA-Tyr/IKVAV-PA hydrogels with arying PA concentrations (0.1, 1, 2 % wt.) were assessed using alcein-AM and Ethidium Homodimer-1 (EthD-1) (Thermo Scienific, USA) staining as well as MTS assay (Biological Industries, USA) t various time points (detailed information is provided in SI).

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO ® -like Bricks.
    Article Snippet: Dead cells were stained with ethidium homodimer-1 (EthD-1; Molecular Probes, Invitrogen, Waltham, USA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation
    Article Snippet: A 10 μL aliquot of the diluted semen sample was combined with 10 μL of 4.65 μM EthD-1 (Ethidium Homodimer-1) (Thermo Fisher Scientific, USA) and 2.7 μL of the SYBR-14 working solution (1:100 v/v in DMSO) and then incubated in a block heater at 37°C for 15 min. One drop (8 μL) of the stained sperm sample was placed onto a glass slide and covered with a coverslip.

    Article Title: Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration
    Article Snippet: Hoechst33342 (Cat# H3570) and ethidium homodimer-1 (EthD-1) (Cat# E1169) were obtained from Thermo Fisher Scientific Inc., (Waltham, MA, USA) and were used to stain nuclear fraction.

    Article Title: A Modular Microfluidic Organoid Platform Using LEGO‐Like Bricks
    Article Snippet: Dead cells were stained with ethidium homodimer‐1 (EthD‐1; Molecular Probes, Invitrogen, Waltham, MA), whereas all cells were counterstained with Hoechst (H3570, Thermo Fisher Scientific) according to manufacturer's instructions.



    Similar Products

    ethidium homodimer-1 (ethd-1)  (Thermo Fisher)
    90
    Thermo Fisher ethidium homodimer-1 (ethd-1)
    Ethidium Homodimer 1 (Ethd 1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/ethidium+homodimer+1/pm40662251-207-6-12
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-1 (ethd-1) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    calcein am ethidium homodimer 1 ethd 1 live  (Biotium)
    95
    Biotium calcein am ethidium homodimer 1 ethd 1 live
    Calcein Am Ethidium Homodimer 1 Ethd 1 Live, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/Ethidium+Homodimer+I/pm41469447-235-15-21
    Average 95 stars, based on 1 article reviews
    calcein am ethidium homodimer 1 ethd 1 live - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    ethidium homodimer 1 ethd 1  (Biotium)
    95
    Biotium ethidium homodimer 1 ethd 1
    Ethidium Homodimer 1 Ethd 1, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/Ethidium+Homodimer+I/10__1016_slash_j__colsurfb__2025__115058-150-36-12
    Average 95 stars, based on 1 article reviews
    ethidium homodimer 1 ethd 1 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    ethidium homodimer-1 ethd-1  (Abbkine Inc)
    90
    Abbkine Inc ethidium homodimer-1 ethd-1
    Ethidium Homodimer 1 Ethd 1, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/ethidium+homodimer+1++ethd+1/pmc11181140__pnas__2404668121__sapp-6-0-8
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-1 ethd-1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ethidium homodimer 1 ethd 1  (MedChemExpress)
    94
    MedChemExpress ethidium homodimer 1 ethd 1
    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
    Ethidium Homodimer 1 Ethd 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/Ethidium+homodimer/pmc12235857-128-15-18
    Average 94 stars, based on 1 article reviews
    ethidium homodimer 1 ethd 1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    ethidium homodimer-1 ethd-1  (Thermo Fisher)
    90
    Thermo Fisher ethidium homodimer-1 ethd-1
    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
    Ethidium Homodimer 1 Ethd 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/ethidium+homodimer+1/10__1016_slash_j__jff__2025__106944-77-4-12
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-1 ethd-1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    calcein am ethidium homodimer 1 ethd 1 kit  (Biotium)
    96
    Biotium calcein am ethidium homodimer 1 ethd 1 kit
    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
    Calcein Am Ethidium Homodimer 1 Ethd 1 Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/Calcein+AM/pm40403819-63-35-41
    Average 96 stars, based on 1 article reviews
    calcein am ethidium homodimer 1 ethd 1 kit - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    calcein am, ethidium homodimer (ethd-1), and live/dead bac light bacterial viability kits  (Thermo Fisher)
    90
    Thermo Fisher calcein am, ethidium homodimer (ethd-1), and live/dead bac light bacterial viability kits
    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
    Calcein Am, Ethidium Homodimer (Ethd 1), And Live/Dead Bac Light Bacterial Viability Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer-1+(ethd-1)/live+dead+fixable+aqua+dead+cell+stain+kit/pmc12078748-54-2-15
    Average 90 stars, based on 1 article reviews
    calcein am, ethidium homodimer (ethd-1), and live/dead bac light bacterial viability kits - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by Calcein-AM/EthD-1 staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Single-cell transcriptome analysis reveals the malignant characteristics of tumour cells and the immunosuppressive landscape in HER2-positive inflammatory breast cancer

    doi: 10.1186/s13046-025-03454-z

    Figure Lengend Snippet: B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by Calcein-AM/EthD-1 staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment

    Article Snippet: HUVECs from control and experimental groups were incubated with 2 μM Calcein-AM and 4 μM Ethidium Homodimer-1 (EthD-1) (MCE, HY-D0093) for 10 min in the dark.

    Techniques: Expressing, Comparison, Staining, Blocking Assay, Immunofluorescence, Imaging, Microscopy, Multiplex Assay, Co-Culture Assay, Western Blot, Cell Culture